oleyl alcohols and Engkabang Fat, utilizing enzyme as biocatalysts in the presence of organic solvents. Three interactive parameters effects, namely temperature-substrate molar ratio effects, impeller speed-enzyme amount effects and substrate molar ratio-enzyme amount effects on Engkabang Fat Esters

Engkabang Fat Esters are produced from the alcoholysis reaction of oleyl alcohol and its fat using enzyme as biocatalyst. The work has been previously carried out mainly on the optimization of Engkabang Fat esters production in organic solvents. However, the effect of impeller speed-substrate molar ratio has yet to be discussed in detail. In the present paper, the influences of impeller speed and substrate molar ratio were further investigated using the model generated by Response Surface Methodology (RSM). The maximum yield for reaction conducted in heptane was observed more than 90% at substrate molar ratio of 2.95 mmol at any impeller speed from 150 to 350 rpm. Meanwhile for hexane, highest yield (85%) was obtained at highest substrate molar ratio (4.00 mmol) and highest impeller speed (350 rpm). Keywords— Engkabang Fat Esters, enzyme, substrate molar ratio, impeller speed